The test for a non-reducing sugar requires boiling with dilute acid first because

  1. the acid denatures proteins
  2. the acid hydrolyses glycosidic bonds to release reducing sugars
  3. the acid makes Benedict's blue
  4. the acid emulsifies lipids
Answer

B) the acid hydrolyses glycosidic bonds to release reducing sugars

Hydrolysis forms monosaccharides that reduce Benedict's. AS & A Level Biology · Topic 2 Biological Molecules · 2.1 Testing for biological molecules

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